RADAR will generate a reliable and robust biosensor device to monitor multiple EDCs on site using the following approach

 

 

Biosensor Engineering and Selection

A good biosensor for a compound is often found by chance, RADAR will use a rational design approach using protein-sequences from aquatic organisms as a basis for modelling and in vitro binding assays to complement and develop the design. Recombinant proteins will be developed to produce modular biosensor components available at large scale and easily purified.

 

 

Label-free detection system

Detection of the EDCs is best done using label-free technologies where the binding of the biosensor receptor and the EDC is observed directly to provide a real-time, quantifiable measurement. Two technologies will be trialled and compared by RADAR, surface plasmon resonance and a proprietary approach.

 

 

Generation of integrated and automated sample preparation module

The sample preparation module for water-based samples needs to remove particles and air bubbles and then concentrate the target compounds for detection by the sensor. Solid samples will have an additional extraction step. CSEM have developed the following approach:

  • Macro-filtration (11μm): removal of debris such as leaves and small organisms - Mechanical filtration
  • Micro-filtration (2.5 μm): removal of large particles - Deterministic Lateral Displacement (DLD) technology
  • Extraction, separation and pre-concentration of the compounds: final preparation for biosensor binding - Solid Phase Extraction

 

 

 

Design and assembly of device with wireless communication capability

Adaptation of existing instrument devices will be required to produce a robust cartridge that can be used in a continuous measurement mode and produced as a disposable or monthly-replacement format. The device must be small, low maintenance and be capable of self calibration

The results produced may need to be transmitted remotely as the monitoring may take place at isolated locations (e.g. at sea) or as part of a busy food process line where disruptions are costly so a wireless communication capability will be incorporated for a rapid response to possible contamination

 

 

Demonstration of utility in real-life situations

The RADAR instruments will be tested at different sites across the Netherlands, UK and Slovenia and will be used for a range of sample types including: sea and fresh water; fish and water from fish farms; milk and fruit juice from processing plants. The RADAR product will be tested for use by non-experts in real-life situations, ensuring it is fit for purpose for further commercial exploitation.

RADAR will generate a reliable and robust biosensor device to monitor multiple EDCs on site using the following approach